Article
A reusable method for construction of non-marker large fragment deletion yeast auxotroph strains: A practice in Torulopsis glabrata.
Journal of microbiological methods - 1 Jan 2009
Zhou Jingwen, Dong Zhiyao, Liu Liming, Du Guocheng, Chen Jian
Abstract excerpt
A reusable method for construction of yeast auxotrophic mutants with large deletions that do not contain markers was developed and successfully applied to a pyruvate overproducing yeast strain, Torulopsis glabrata CCTCC M202019. A URA3 knockout fragment with a large deletion of the open reading frame and long homologous arms was constructed by fusion PCR and transformed into the parent strain by high-efficiency...
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