Article
Transient reversal of RNA polymerase II active site closing controls fidelity of transcription elongation.
Molecular cell - 6 Jun 2008
Kireeva Maria L, Nedialkov Yuri A, Cremona Gina H, Purtov Yuri A, Lubkowska Lucyna, Malagon Francisco, Burton Zachary F, Strathern Jeffrey N, Kashlev Mikhail
Abstract excerpt
To study fidelity of RNA polymerase II (Pol II), we analyzed properties of the 6-azauracil-sensitive and TFIIS-dependent E1103G mutant of rbp1 (rpo21), the gene encoding the catalytic subunit of Pol II in Saccharomyces cerevisiae. Using an in vivo retrotransposition-based transcription fidelity assay, we observed that rpb1-E1103G causes a 3-fold increase in transcription errors. This mutant showed a 10-fold...
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