Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

A genetic assay for transcription errors reveals multilayer control of RNA polymerase II fidelity.

PLoS genetics - 1 Sept 2014

Irvin Jordan D, Kireeva Maria L, Gotte Deanna R, Shafer Brenda K, Huang Ingold, Kashlev Mikhail, Strathern Jeffrey N

Abstract excerpt

We developed a highly sensitive assay to detect transcription errors in vivo. The assay is based on suppression of a missense mutation in the active site tyrosine in the Cre recombinase. Because Cre acts as tetramer, background from translation errors are negligible. Functional Cre resulting from rare transcription errors that restore the tyrosine codon can be detected by Cre-dependent rearrangement of reporter...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Catalytic Domain
Share to Codon

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Expression Regulation, Fungal

Share to Genes, Reporter

Share to RNA Polymerase II

Share to Saccharomyces cerevisiae

Share to Saccharomyces cerevisiae Proteins

Share to Transcription, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 25232834
DOI: 10.1371/journal.pgen.1004532
PMCID: PMC4168980

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

A genetic assay for transcription errors reveals multilayer control of RNA polymerase II fidelity.PMID 25232834Read ↗

Isolation and characterization of transcription fidelity mutants.Semantic proximity Read ↗

Lost in transcription: transient errors in information transfer.Semantic proximity Read ↗

The fidelity of transcription: RPB1 (RPO21) mutations that increase transcriptional slippage in S. cerevisiae.Semantic proximity Read ↗

Transient reversal of RNA polymerase II active site closing controls fidelity of transcription elongation.Semantic proximity Read ↗

The landscape of transcription errors in eukaryotic cells.Semantic proximity Read ↗

RNA polymerase errors cause splicing defects and can be regulated by differential expression of RNA polymerase subunits.Semantic proximity Read ↗

High-Resolution Phenotypic Landscape of the RNA Polymerase II Trigger Loop.Semantic proximity Read ↗

A Genome-Wide Assay Specifies Only GreA as a Transcription Fidelity Factor in Escherichia coli.Semantic proximity Read ↗

Transcription errors induce proteotoxic stress and shorten cellular lifespan.Semantic proximity Read ↗

Mutations in a conserved region of RNA polymerase II influence the accuracy of mRNA start site selection.Semantic proximity Read ↗

Transcription-coupled DNA repair in yeast transcription factor IIE (TFIIE) mutants.Semantic proximity Read ↗

Heritable change caused by transient transcription errors.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.