Article
Specificity of the polioviral proteinase 3C towards genetically engineered cleavage sites in the viral capsid.
The Journal of general virology - 1 May 1991
Mirzayan C, Ingraham R, Wimmer E
Abstract excerpt
In a study of the cleavage specificity of poliovirus proteinase 3Cpro, two mutant polioviruses were constructed to include putative 3Cpro cleavage sites in the BC loop of VP1. The BC loop of VP1 in the wild-type virus is the neutralization antigenic site IA, consisting of a continuous chain of ni...
Topics
- 3C Viral Proteases
- Amino Acid Sequence
- Capsid
- Cysteine Endopeptidases
- Electrophoresis, Polyacrylamide Gel
- Genetic Engineering
- HeLa Cells
- Humans
- Hydrolysis
- Molecular Sequence Data
- Mutation
- Poliovirus
- Substrate Specificity
- Viral Proteins