Article
Stem cell transcriptome profiling via massive-scale mRNA sequencing.
Nature methods - 1 Jul 2008
Cloonan Nicole, Forrest Alistair R R, Kolle Gabriel, Gardiner Brooke B A, Faulkner Geoffrey J, Brown Mellissa K, Taylor Darrin F, Steptoe Anita L, Wani Shivangi, Bethel Graeme, Robertson Alan J, Perkins Andrew C, Bruce Stephen J, Lee Clarence C, Ranade Swati S, Peckham Heather E, Manning Jonathan M, McKernan Kevin J, Grimmond Sean M
Abstract excerpt
We developed a massive-scale RNA sequencing protocol, short quantitative random RNA libraries or SQRL, to survey the complexity, dynamics and sequence content of transcriptomes in a near-complete fashion. This method generates directional, random-primed, linear cDNA libraries that are optimized for next-generation short-tag sequencing. We surveyed the poly(A)(+) transcriptomes of undifferentiated mouse embryonic...
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