Article

Enhancing Transcriptome Mapping with Rapid PRO-seq Profiling of Nascent RNA

2024-05-10

Abstract excerpt

Precision nuclear run-on (PRO) sequencing (PRO-seq) is a powerful technique for mapping polymerase active sites with nucleotide resolution and measuring newly synthesized transcripts at both promoters and enhancer elements. The current PRO-seq protocol is time-intensive, technically challenging, and requires a large amount of starting material. To overcome these limitations, we developed rapid PRO-seq (rPRO-seq) w...

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Literature Corpus work: 83e18cf1-f9a4-5644-b059-d6cd9f78b01d
DOI: 10.1101/2024.05.08.593182

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