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Article

Transcriptional profiling of cells sorted by RNA abundance.

Nature methods - 1 May 2014

Klemm Sandy, Semrau Stefan, Wiebrands Kay, Mooijman Dylan, Faddah Dina A, Jaenisch Rudolf, van Oudenaarden Alexander

Abstract excerpt

We have developed a quantitative technique for sorting cells on the basis of endogenous RNA abundance, with a molecular resolution of 10-20 transcripts. We demonstrate efficient and unbiased RNA extraction from transcriptionally sorted cells and report a high-fidelity transcriptome measurement of mouse induced pluripotent stem cells (iPSCs) isolated from a heterogeneous reprogramming culture. This method is...

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Identifiers

PMID: 24681693
DOI: 10.1038/nmeth.2910
PMCID: PMC4174458

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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