Article
Profiling the HeLa S3 transcriptome using randomly primed cDNA and massively parallel short-read sequencing.
BioTechniques - 1 Jul 2008
Morin Ryan, Bainbridge Matthew, Fejes Anthony, Hirst Martin, Krzywinski Martin, Pugh Trevor, McDonald Helen, Varhol Richard, Jones Steven, Marra Marco
Abstract excerpt
Sequence-based methods for transcriptome characterization have typically relied on generation of either serial analysis of gene expression tags or expressed sequence tags. Although such approaches have the potential to enumerate transcripts by counting sequence tags derived from them, they typically do not robustly survey the majority of transcripts along their entire length. Here we show that massively parallel...
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