Article
Genetic tools used for cell lineage tracing and gene manipulation in Drosophila germline stem cells.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2008
Liu Wei, Hou Steven X
Abstract excerpt
The advancement of Drosophila germline stem cell research accompanies the development of powerful new tools for genetic analysis. These include the techniques of stem cell labeling, cell lineage tracing, mosaic mutant analysis, and gene manipulation in targeted cell populations, which together constitute the critical methodologies in stem cell research. We discuss four such techniques: the tubulin-lacZ...
Topics
- Adult Stem Cells
- Animals
- Animals, Genetically Modified
- Drosophila
- Female
- Genetic Techniques
- Germ Cells
- Green Fluorescent Proteins
- Lac Operon
- Male
- Mosaicism
- Mutation
- Recombinant Proteins
- Repressor Proteins
- Saccharomyces cerevisiae Proteins
- Tubulin
