Article
Generation of standard wild-type MARCM clones for analysis of drosophila brain development.
Cold Spring Harbor protocols - 1 Dec 2012
Kao Chih-Fei, Lee Tzumin
Abstract excerpt
The generation and analysis of clones of cells with a genotype different from the rest of an organism has been used for studying molecular mechanisms underlying development. Genetic mosaics in Drosophila typically involve derivation of homozygous daughter cells from heterozygous precursors through mitotic recombination. MARCM (mosaic analysis with a repressible cell marker) couples loss of heterozygosity with...
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