Article
Mutant analysis by rescue gene excision: New tools for mosaic studies in Drosophila.
Genesis (New York, N.Y. : 2000) - 1 Nov 2016
Zhou Qingxiang, Neal Scott J, Pignoni Francesca
Abstract excerpt
A host of classical and molecular genetic tools make Drosophila a tremendous model for the dissection of gene activity. In particular, the FLP-FRT technique for mitotic recombination has greatly enhanced gene loss-of-function analysis. This technique efficiently induces formation of homozygous mutant clones in tissues of heterozygous organisms. However, the dependence of the FLP-FRT method on cell division, and...
Topics
- Animals
- DNA Mutational Analysis
- Drosophila
- Drosophila Proteins
- Female
- Homozygote
- Imaginal Discs
- Mosaicism
- Nuclear Proteins
- Ovary
- Phenotype
- Recombination, Genetic
- Trans-Activators
- YAP-Signaling Proteins