Using MARCM to study Drosophila brain development.

doi:10.1007/978-1-62703-655-9_6

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Article

Using MARCM to study Drosophila brain development.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014

Viktorin Gudrun

Abstract excerpt

Mosaic analysis with a repressible cell marker (MARCM) generates positively labeled, wild-type or mutant mitotic clones by unequally distributing a repressor of a cell lineage marker, originally tubP-driven GAL80 repressing the GAL4/UAS system. Variations of the technique include labeling of both sister clones (twin spot MARCM), the simultaneous use of two different drivers within the same clone (dual MARCM), as...

Topics

Animals
Biomarkers
Brain
Clone Cells
Cytological Techniques
Drosophila melanogaster
Female
Hybridization, Genetic
Immunohistochemistry
Male
Microscopy
Mitosis
Mutation
Spatio-Temporal Analysis
Staining and Labeling
Tissue Fixation
Transcription, Genetic

Source

Open in PubMed

Identifiers

PMID: 24048928
DOI: 10.1007/978-1-62703-655-9_6

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