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Article

Highly efficient deletion method for the engineering of plasmid DNA with single-stranded oligonucleotides.

BioTechniques - 1 Feb 2008

Lu Lin-Yu, Huen Michael S Y, Tai Andrew Chi-Pang, Liu De-Pei, Cheah Kathryn S E, Huang Jian-Dong

Abstract excerpt

The lamda phage Red recombination system has been used to modify plasmid, bacterial artificial chromosome (BAC), and chromosomal DNA in a highly precise and versatile manner Linear double-stranded DNA fragments or synthetic single-stranded oligonucleotides (SSOs) with short flanking homologies (<50 bp) to the target loci can be used as substrates to direct changes, including point mutations, insertions, and...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 18330349
DOI: 10.2144/000112684

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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