Article
Isolation of Chinese hamster ovary cell lines producing Man3GlcNAc2 asparagine-linked glycans.
Analytical biochemistry - 2 Mar 1991
Zeng Y, Lehrman M A
Abstract excerpt
Chinese hamster ovary lines with two mutations, one causing accumulation of Man5GlcNAc2-P-P-dolichol and a second resulting in defective N-acetylglucosaminyltransferase I activity, synthesize asparagine-linked glycans with the structure Man3GlcNAc2. As a result, the asparagine-linked glycans produced by these lines are smaller and less heterogeneous than those produced by other currently available animal cell lines.
Topics
- Animals
- Carbohydrate Sequence
- Cell Line
- Chromatography, High Pressure Liquid
- Cricetinae
- Cricetulus
- Genotype
- Glucosyltransferases
- Molecular Sequence Data
- N-Acetylglucosaminyltransferases
- Polyisoprenyl Phosphate Oligosaccharides