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Isolation of new CHO cell mutants defective in CMP-sialic acid biosynthesis and transport.

Molecules and cells - 31 Dec 2006

Shin Dong-Jun, Kang Ji Young, Kim Youn Uck, Yoon Joong Sik, Choy Hyon E, Maeda Yusuke, Kinoshita Taroh, Hong Yeongjin

Abstract excerpt

Sialic acid is a sugar typically found at the N-glycan termini of glycoproteins in mammalian cells. Lec3 CHO cell mutants are deficient in epimerase activity, due to a defect in the gene that encodes a bifunctional UDP-GlcNAc 2-epimerase/ManNAc kinase (GNE). Sialic acid modification on the cell surface is partially affected in these cells. We have mutagenized Lec3 CHO cells and isolated six mutants (termed C2m)...

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PMID: 17202864

Genes

GNE

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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