Article
alpha-Glucosidase II-deficient cells use endo alpha-mannosidase as a bypass route for N-linked oligosaccharide processing.
The Journal of biological chemistry - 25 Feb 1991
Fujimoto K, Kornfeld R
Abstract excerpt
The kinetics of N-linked oligosaccharide processing and the structures of the processing intermediates have been examined in normal parental BW5147 mouse lymphoma cells and the alpha-glucosidase II-deficient PHAR2.7 mutant cells. The mutant cells accumulated glucosylated intermediates but were able to deglucosylate and process about 40% of their oligosaccharides to complex-type. This processing was not due to...
Topics
- 1-Deoxynojirimycin
- Animals
- Chromatography, Affinity
- Chromatography, Paper
- Glucosamine
- Glycoside Hydrolase Inhibitors
- Kinetics
- Lymphoma
- Mannosidases
- Mice
- Mutation