Article
Gain-of-function Chinese hamster ovary mutants LEC18 and LEC14 each express a novel N-acetylglucosaminyltransferase activity.
The Journal of biological chemistry - 5 Jun 1998
Raju T S, Stanley P
Abstract excerpt
LEC18 and LEC14 cells are gain-of-function glycosylation mutants isolated from Chinese hamster ovary cells for resistance to pea lectin. Structural studies have shown that LEC18 cells synthesize complex N-glycans with a GlcNAc residue linked at the O-6 position of the core GlcNAc (Raju, T. S., Ra...
Topics
- Acetylglucosamine
- Adenosine Triphosphate
- Animals
- CHO Cells
- Carbohydrate Conformation
- Carbohydrate Sequence
- Cricetinae
- Glycopeptides
- Hydrogen-Ion Concentration
- Kinetics
- Manganese
- Molecular Sequence Data
- Mutation
- N-Acetylglucosaminyltransferases
- Oligosaccharides
- Polysaccharides
- Substrate Specificity
- beta-N-Acetylhexosaminidases