Article
Preliminary characterization of a Chinese hamster ovary cell glycosylation mutant isolated by screening for low intracellular lysosomal enzyme activity.
Molecular and cellular biochemistry - 1 Jan 2000
Hall C W, Robbins A R, Krag S S
Abstract excerpt
A novel screening procedure was developed for isolating Chinese hamster ovary cell mutants altered in the early steps of the biosynthesis of asparagine-linked glycoproteins. This procedure identifies cells with low intracellular levels of two lysosomal hydrolases, beta-glucuronidase and alpha-idu...
Topics
- Animals
- Carrier Proteins
- Cell Line
- Cricetinae
- Glucuronidase
- Glycoproteins
- Glycosylation
- Iduronidase
- Lysosomes
- Mannosephosphates
- Mutation
- Protein Processing, Post-Translational
- Receptor, IGF Type 2