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Article

New pSC101-derivative cloning vectors with elevated copy numbers.

Plasmid - 1 May 2008

Peterson James, Phillips Gregory J

Abstract excerpt

Mutations that increase the copy number of the pSC101 replicon have been used for construction of new cloning vectors. Replacement of glutamate at position 93 in RepA yields plasmids that replicate at medium (27 copies/cell) and high (approximately 240 copies/cell) copy numbers. Based on the crystal structure of RepE, a structurally similar replication initiator protein from the F factor, the pSC101 repA mutants...

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Identifiers

PMID: 18295880
DOI: 10.1016/j.plasmid.2008.01.004
PMCID: PMC2435062

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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