Mini-F plasmid mutants able to replicate in the absence of sigma 32: mutations in the repE coding region producing hyperactive initiator protein.

doi:10.1128/jb.173.3.1064-1072.1991

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Article

Mini-F plasmid mutants able to replicate in the absence of sigma 32: mutations in the repE coding region producing hyperactive initiator protein.

Journal of bacteriology - 1 Feb 1991

Kawasaki Y, Wada C, Yura T

Abstract excerpt

Mini-F plasmids cannot replicate in Escherichia coli strains (delta rpoH) lacking sigma 32, presumably because transcription of the repE gene encoding the replication initiator protein (RepE protein) depends mostly on RNA polymerase containing sigma 32. We have isolated and characterized mini-F mutants able to replicate in delta rpoH cells. Contrary to the initial expectation, five mutants with mutations in the...

Topics

Amino Acid Sequence
Bacterial Proteins
Base Sequence
Cloning, Molecular
DNA Helicases
DNA Replication
DNA, Bacterial
DNA-Binding Proteins
Escherichia coli
F Factor
Genes, Bacterial

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Immunoblotting

Molecular Sequence Data

Mutation

Restriction Mapping

Sigma Factor

Trans-Activators

Source

Open in PubMed Open full text

Identifiers

PMID: 1991708
DOI: 10.1128/jb.173.3.1064-1072.1991
PMCID: PMC207225

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1991708-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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