Article
Mini-F plasmid mutants able to replicate in Escherichia coli deficient in the DnaJ heat shock protein.
Journal of bacteriology - 1 Sept 1992
Ishiai M, Wada C, Kawasaki Y, Yura T
Abstract excerpt
A subset of Escherichia coli heat shock proteins, DnaJ, DnaK, and GrpE, is required for mini-F plasmid replication, presumably at the step of functioning of the RepE initiator protein. We have isolated and characterized mini-F plasmid mutants that acquired the ability to replicate in the Escherichia coli dnaJ259. The mutant plasmids were found to replicate in any of dnaJ, dnaK, and grpE mutant hosts tested. In...
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