Article
A copy-number mutant of plasmid pSC101.
Molecular microbiology - 1 Mar 1991
Xia G X, Manen D, Goebel T, Linder P, Churchward G, Caro L
Abstract excerpt
Copy-number mutants of plasmid pSC101 were isolated by u.v. mutagenesis and selection for elevated expression of ampicillin resistance. Three independent mutations were identical and mapped in codon 93 of the initiation protein RepA. The mutated plasmids were maintained at a level four to five ti...
Topics
- Ampicillin Resistance
- Bacterial Proteins
- Base Sequence
- DNA Helicases
- DNA Replication
- DNA-Binding Proteins
- Escherichia coli
- Gene Expression
- Molecular Sequence Data
- Mutation
- Plasmids
- Proteins
- RNA, Bacterial
- RNA, Messenger
- Repetitive Sequences, Nucleic Acid
- Salmonella
- Trans-Activators