Article
Analysis of a copy number mutant of plasmid pSC101: co-maintenance of wild type and mutant plasmids.
Research in microbiology - 1 Jan 2000
Goebel T, Manen D, Alff-Steinberger C, Xia G X, Caro L
Abstract excerpt
We have isolated a high copy number mutant of plasmid pSC101 which is maintained at a level 4 times higher than that of the wild type. The mutation is a single base change that maps in codon 93 of the initiation protein RepA. We find that the mutation relaxes the autoregulation of the protein but increases its affinity for the repeated sequences in the origin. The wild type and the mutant repA genes are...
Topics
- Bacterial Proteins
- Computer Simulation
- DNA Helicases
- DNA Replication
- DNA-Binding Proteins
- Escherichia coli
- Gene Amplification
- Genes, Bacterial
- Mutation
- Plasmids
- Proteins