Article
Effects of mutation at the D-JH junction on affinity, specificity, and idiotypy of anti-progesterone antibody DB3.
Protein science : a publication of the Protein Society - 1 Sept 2006
He Mingyue, Hamon Maureen, Liu Hong, Corper Adam L, Taussig Michael J
Abstract excerpt
The crystal structures of the Fab' fragment of the anti-progesterone monoclonal antibody DB3 and its complexes with steroid haptens have shown that the D-JH junctional residue TrpH100 is a key contributor to binding site interactions with ligands. The indole group of TrpH100 also undergoes a significant conformational change between the bound and unliganded states, effectively opening and closing the combining...
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