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Article

A single active site mutation inverts stereoselectivity of 16-hydroxylation of testosterone catalyzed by engineered cytochrome P450 BM3.

Chembiochem : a European journal of chemical biology - 5 Mar 2012

Venkataraman Harini, Beer Stephanie B A de, Bergen Laura A H van, Essen Nick van, Geerke Daan P, Vermeulen Nico P E, Commandeur Jan N M

Abstract excerpt

Inversion of stereoselectivity: screening of a minimal mutant library revealed a cytochrome P450 BM3 variant M01 A82W S72I capable of producing 16 α-OH-testosterone. Remarkably, a single active site mutation S72I in M01 A82W inverted the stereoselectivity of hydroxylation from 16 β to 16 α. Introduction of S72I mutation in another 16 β-OH-selective variant M11 V87I, also resulted in similar inversion of...

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PMID: 22275147
DOI: 10.1002/cbic.201100750

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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