Article
Generation and characterization of an immunogenic dendritic cell population.
Journal of immunological methods - 20 Mar 2008
Gehring Stephan, Gregory Stephen H, Wintermeyer Philip, San Martin Maryann, Aloman Costica, Wands Jack R
Abstract excerpt
Dendritic cells (DCs) capture, internalize and process antigens leading to the induction of antigen-specific immune responses. The aim of this study was to develop, implement and characterize an efficient approach for DC-based immunization. Dendritic cells were expanded in vivo by hydrodynamic delivery of a human flt3 ligand expression plasmid. Splenic DCs were isolated and purified with magnetic beads linked to...
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