Article
Immunolipoplexes: an efficient, nonviral alternative for transfection of human dendritic cells with potential for clinical vaccination.
Molecular therapy : the journal of the American Society of Gene Therapy - 1 May 2005
Tan Peng H, Beutelspacher Sven C, Wang Yao-He, McClure Myra O, Ritter Mary A, Lombardi Giovanna, George Andrew J T
Abstract excerpt
Genetic manipulation of dendritic cells (DCs) is important in the context of using either mature DCs to immunize patients or immature DCs to induce tolerance. Here, we describe a novel method of transfecting monocyte-derived human DCs using immunolipoplexes containing anti-CD71 or anti-CD205 monoclonal Abs. This results in up to 20% transfection, which can be increased to 20-30% if the immunolipoplexes are used...
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