Article
In vitro generation of murine myeloid dendritic cells from CD34-positive precursors.
Cell biology international - 1 Jul 2009
Stephen Tom-Li, Harms Fabian, Fabri Mario, Flenner Eva, Bessler Martina, Hafke Helena, Meemboor Sonja, Kalka Christoph, Kalka-Moll Wiltrud
Abstract excerpt
Dendritic cells (DCs) link the innate and adaptive immune system. Currently, murine DCs for cell biology investigations are developed from MHC class II-negative bone marrow (BM) precursor cells, non-depleted BM cells or BM monocytes in the presence of granulocyte-macrophage colony-stimulating factor (GM-CSF). Here we demonstrate an isolation procedure of functionally intact myeloid CD11c(+) CD11b(+) DCs derived...
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