Article
Ligand-specific changes in M3 muscarinic acetylcholine receptor structure detected by a disulfide scanning strategy.
Biochemistry - 4 Mar 2008
Li Jian Hua, Hamdan Fadi F, Kim Soo-Kyung, Jacobson Kenneth A, Zhang Xiaohong, Han Sung-Jun, Wess Jürgen
Abstract excerpt
G protein-coupled receptor (GPCR) function can be modulated by different classes of ligands including full and inverse agonists. At present, little is known about the conformational changes that agonist ligands induce in their target GPCRs. In this study, we employed an in situ disulfide cross-linking strategy to monitor ligand-induced structural changes in a series of cysteine (Cys)-substituted mutant M 3...
Topics
- Amino Acid Sequence
- Animals
- Blotting, Western
- COS Cells
- Carbachol
- Chlorocebus aethiops
- Disulfides
- Hydrolysis
- Ligands
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Phosphatidylinositols
- Protein Conformation