Article
Identification of an agonist-induced conformational change occurring adjacent to the ligand-binding pocket of the M(3) muscarinic acetylcholine receptor.
The Journal of biological chemistry - 14 Oct 2005
Han Sung-Jun, Hamdan Fadi F, Kim Soo-Kyung, Jacobson Kenneth A, Bloodworth Lanh M, Li Bo, Wess Jürgen
Abstract excerpt
To study the conformational changes that convert G protein-coupled receptors (GPCRs) from their resting to their active state, we used the M(3) muscarinic acetylcholine receptor, a prototypical class A GPCR, as a model system. Specifically, we employed a recently developed in situ disulfide cross-linking strategy that allows the formation of disulfide bonds in Cys-substituted mutant M(3) muscarinic receptors...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.