Article
Conformational thermostabilization of the beta1-adrenergic receptor in a detergent-resistant form.
Proceedings of the National Academy of Sciences of the United States of America - 22 Jan 2008
Serrano-Vega Maria J, Magnani Francesca, Shibata Yoko, Tate Christopher G
Abstract excerpt
There are approximately 350 non-odorant G protein-coupled receptors (GPCRs) encoded by the human genome, many of which are predicted to be potential therapeutic targets, but there are only two structures available to represent the whole of the family. We hypothesized that improving the detergent stability of these receptors and simultaneously locking them into one preferred conformation will greatly improve the...
Topics
- Detergents
- Models, Molecular
- Mutation
- Protein Denaturation
- Protein Structure, Tertiary
- Receptors, Adrenergic, beta-1
- Temperature