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Article

A mutagenesis and screening strategy to generate optimally thermostabilized membrane proteins for structural studies.

Nature protocols - 1 Aug 2016

Magnani Francesca, Serrano-Vega Maria J, Shibata Yoko, Abdul-Hussein Saba, Lebon Guillaume, Miller-Gallacher Jennifer, Singhal Ankita, Strege Annette, Thomas Jennifer A, Tate Christopher G

Abstract excerpt

The thermostability of an integral membrane protein (MP) in detergent solution is a key parameter that dictates the likelihood of obtaining well-diffracting crystals that are suitable for structure determination. However, many mammalian MPs are too unstable for crystallization. We developed a thermostabilization strategy based on systematic mutagenesis coupled to a radioligand-binding thermostability assay that...

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Identifiers

PMID: 27466713
DOI: 10.1038/nprot.2016.088
PMCID: PMC5268090

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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