Article
The roles of amino acid residues at positions 216 and 219 in the structural stability and metabolic functions of rat cytochrome P450 2D1 and 2D2.
Chemico-biological interactions - 10 Mar 2008
Narimatsu Shizuo, Kiryu Kimio, Yonemoto Rei, Yoshino Manabu, Kobatake Mitsuko, Kazamori Daichi, Hagino Saori, Masuda Kazufumi, Katsu Takashi, Asanuma Masato, Kumamoto Takuya, Ishikawa Tsutomu, Funae Yoshihiko, Yamano Shigeru, Hanioka Nobumitsu, Naito Shinsaku
Abstract excerpt
We examined the effects of the mutual substitution of amino acid residues at positions 216 and 219 between rat CYP2D1 and CYP2D2 on their microsomal contents and enzymatic functions using a yeast cell expression system and 5-methoxy-N,N-diisopropyltryptamine (5-MeO-DIPT) as a substrate. CYP2D1 has amino acid residues, leucine and valine, at positions of 216 and 219, respectively, whereas CYP2D2 has phenylalanine...
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