Article
Role of the alanine at position 363 of cytochrome P450 2B2 in influencing the NADPH- and hydroperoxide-supported activities.
Archives of biochemistry and biophysics - 15 Feb 1998
Hanna I H, Teiber J F, Kokones K L, Hollenberg P F
Abstract excerpt
Escherichia coli was used to express the two closely related cytochromes P450 2B1 and 2B2 and two mutants of 2B2 in which residues Gly-303 and Ala-363 were replaced by Ser and Val, respectively. The expressed proteins were partially purified and assayed for benzphetamine and n-octylamine (NOA) bi...
Topics
- 9,10-Dimethyl-1,2-benzanthracene
- Amines
- Animals
- Aryl Hydrocarbon Hydroxylases
- Benzene Derivatives
- Benzphetamine
- Coumarins
- Cytochrome P-450 CYP2B1
- Cytochrome P-450 Enzyme System
- Cytochrome b Group
- Hydroxylation
- Microsomes, Liver
- Mutation
- Protein Binding
- Rats
- Recombinant Proteins
- Sequence Analysis, DNA
- Steroid Hydroxylases