Article
T41 mutation in lac repressor is Tyr282----Asp.
Gene - 1 Feb 1992
Chen J, Matthews K S
Abstract excerpt
A monomeric mutant of the lac repressor protein, designated T41, produced originally by the in vivo MutT mutagenesis exhibited behavior similar to mutants identified as Tyr282----Ser or Tyr282----Gln [Schmitz et al., J. Biol. Chem. 251 (1976) 3359-3366]. The T41 gene, encoded within a phage lambda prophage in the Escherichia coli genome, was amplified by polymerase chain reaction and sequenced. The only mutation...
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