Article
Bacteriophage T7 RNA polymerase and its active-site mutants. Kinetic, spectroscopic and calorimetric characterization.
Journal of molecular biology - 18 Mar 1994
Osumi-Davis P A, Sreerama N, Volkin D B, Middaugh C R, Woody R W, Woody A Y
Abstract excerpt
It has been demonstrated that the amino acids Asp537, Asp812, Lys631, His811 and Tyr639 are involved in bacteriophage T7 RNA polymerase catalysis. In the present paper, we report kinetic, spectroscopic and calorimetric characterization of the wild-type and mutant T7 RNA polymerases generated at t...
Topics
- Amino Acid Sequence
- Bacteriophage T7
- Base Sequence
- Binding Sites
- Calorimetry, Differential Scanning
- Circular Dichroism
- DNA-Directed RNA Polymerases
- Hydrogen-Ion Concentration
- Kinetics
- Molecular Sequence Data
- Mutation
- Protein Conformation
- Protein Structure, Secondary
- Spectrometry, Fluorescence
- Spectrophotometry, Ultraviolet
- Templates, Genetic
- Thermodynamics
- Transcription, Genetic