Article
Use of a competitive probe in assay design for genotyping of the UGT1A1 *28 microsatellite polymorphism by the smart amplification process.
BioTechniques - 1 Oct 2007
Watanabe Jun, Mitani Yasumasa, Kawai Yuki, Kikuchi Takeshi, Kogo Yasushi, Oguchi-Katayama Atsuko, Kanamori Hajime, Usui Kengo, Itoh Masayoshi, Cizdziel Paul E, Lezhava Alexander, Tatsumi Kenji, Ichikawa Yasushi, Togo Shinji, Shimada Hiroshi, Hayashizaki Yoshihide
Abstract excerpt
A key feature of the smart amplification process version 2 (SMAP-2) is the ability to suppress mismatch amplification by using a unique asymmetric primer design and Thermus aquaticus MutS (Taq MutS). However we report here that use of SMAP-2 for polymorphism determination of the UGT1A1 *28 allele...
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