Article
Enhanced specificity of TPMT*2 genotyping using unidirectional wild-type and mutant allele-specific scorpion primers in a single tube.
PloS one - 1 Jan 2014
Chen Dong, Yang Zhao, Xia Han, Huang Jun-Fu, Zhang Yang, Jiang Tian-Nun, Wang Gui-Yu, Chuai Zheng-Ran, Fu Wei-Ling, Huang Qing
Abstract excerpt
Genotyping of thiopurine S-methyltransferase (TPMT) is recommended for predicting the adverse drug response of thiopurines. In the current study, a novel version of allele-specific PCR (AS-PCR), termed competitive real-time fluorescent AS-PCR (CRAS-PCR) was developed to analyze the TPMT*2 genotype in ethnic Chinese. This technique simultaneously uses wild-type and mutant allele-specific scorpion primers in a...
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