Article
Rapid detection of the irinotecan-related UGT1A1*28 polymorphism by asymmetric PCR melting curve analysis using one fluorescent probe.
Journal of clinical laboratory analysis - 1 Aug 2022
Kong Xiaomu, Xu Ye, Gao Peng, Liu Yi, Wang Xuran, Zhao Meimei, Jiang Yongwei, Yang Hui, Cao Yongtong, Ma Liang
Abstract excerpt
BACKGROUND: Determination of UGT1A1 (TA)n polymorphism prior to irinotecan therapy is necessary to avoid severe adverse drug effects. Thus, accurate and reliable genotyping methods for (TA)n polymorphism are highly desired. Here, we present a new method for polymerase chain reaction (PCR) melting curve analysis using one fluorescent probe to discriminate the UGT1A1*1 [(TA)6 ] and *28 [(TA)7 ] genotypes. METHODS:...
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