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Article

HbS-Savaria: the anti-polymerization effect of a single mutation in human alpha-chains.

The protein journal - 1 Dec 2007

Srinivasulu Sonati, Acharya A Seetharama, Prabhakaran Muthuchidambaran, Fabry Mary E, Alami Raouf, Fiering Steven N, Bouhasirra Eric E, Nagel Ronald L

Abstract excerpt

Recombinant alpha-Savaria globin (alpha(S49R)) was assembled with beta(S) chains by the alloplex intermediate pathway to generate tetrameric rHbS-Sarvaria (alpha (2) (S49R) beta (2) (E6V) ) that exhibited normal O(2) affinity and co-operatively at pH 7.4. Allosteric effectors, 2,3-DPG, L35, and NaCl increased O(2) affinity by 15%. Bohr effects were similar for rHbS-Savaria and HbS (0.38 +/- 0.025 vs. 0.46 +/-...

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Identifiers

PMID: 17973182
DOI: 10.1007/s10930-007-9089-9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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