Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Haemoglobin alpha 2 beta 2 23Val----Ile produced in Escherichia coli facilitates Hb S polymerization.

British journal of haematology - 1 Apr 1990

Pagnier J, Baudin-Chich V, Lacaze N, Bohn B, Poyart C

Abstract excerpt

The doubly substituted variant Hb S-Antilles (beta 6 Glu----Val, beta 23 Val----Ile) produces sickling in heterozygous carriers. The Csat value for pure deoxyHb S-Antilles is nearly half that of deoxyHb S. Dilute solutions of pure Hb S-Antilles have a lower oxygen affinity than those of Hb A or Hb S. The mutant Hb alpha 2 beta 2 23 Val----Ile was synthesized in E. coli. It exhibits a decreased oxygen affinity...

Topics

Escherichia coli
Hemoglobin, Sickle
Macromolecular Substances
Mutation
Protein Engineering
Structure-Activity Relationship

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 2189492
DOI: 10.1111/j.1365-2141.1990.tb06346.x

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2189492-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Haemoglobin alpha 2 beta 2 23Val----Ile produced in Escherichia coli facilitates Hb S polymerization.PMID 2189492Read ↗

Role of beta87 Thr in the beta6 Val acceptor site during deoxy Hb S polymerization.Semantic proximity Read ↗

Sickle Cell Hemoglobin with Mutation at αHis-50 Has Improved Solubility.Semantic proximity Read ↗

Roles of alpha 114 and beta 87 amino acid residues in the polymerization of hemoglobin S: implications for gene therapy.Semantic proximity Read ↗

Role of Leu-beta 88 in the hydrophobic acceptor pocket for Val-beta 6 during hemoglobin S polymerization.Semantic proximity Read ↗

Influence of semisynthetic modification of the scaffold of a contact domain of HbS on polymerization: role of flexible surface topology in polymerization inhibition.Semantic proximity Read ↗

A Recombinant Human Hemoglobin with Anti-sickling Properties Greater than Fetal HemoglobinSemantic proximity Read ↗

Effects of different beta73 amino acids on formation of 14-stranded fibers of Hb S versus double-stranded crystals of Hb C-Harlem.Semantic proximity Read ↗

Modification of axial fiber contact residues impact sickle hemoglobin polymerization by perturbing a network of coupled interactions.Semantic proximity Read ↗

Site mutations disrupt inter-helical H-bonds (alpha14W-alpha67T and beta15W-beta72S) involved in kinetic steps in the hemoglobin R-->T transition without altering the free energies of oxygenation.Semantic proximity Read ↗

Biophysical Basis of Hb-S Polymerization in Red Blood Cell SicklingSemantic proximity Read ↗

Recombinant sickle hemoglobin containing a lysine substitution at Asp-85(alpha): expression in yeast, functional properties, and participation in gel formation.Semantic proximity Read ↗

HbS-Savaria: the anti-polymerization effect of a single mutation in human alpha-chains.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.