Surveyor nuclease-based detection of p53 gene mutations in haematological malignancy.

Abstract excerpt

BACKGROUND: Surveyor nuclease is a new single-strand-specific endonuclease that cleaves heteroduplex DNA at a base-mismatch site in both DNA strands. We applied this enzyme to detection of p53 gene mutations in haematological malignancy. METHODS: DNA fragments including exons 5 and 6, and those including exons 7 and 8, of the p53 gene, were amplified by polymerase chain reaction using DNA samples extracted from...

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