Article
Screening of the entire coding region of p53 in low grade lymphoproliferative disorders.
Molecular pathology : MP - 1 Aug 2000
Bromidge T J, Howe D J
Abstract excerpt
This report details a rapid method for screening the entire p53 coding region (exons 2-11). This method, based on the non-isotopic RNase cleavage assay, uses novel primer sequences and an adaptation of the MutationScreener method. A mutation in 20% of the sample was easily detectable by this method, whereas mutations below 50% were undetectable using the original method. Alterations to the wild-type p53 mRNA...
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