Article
Highly efficient somatic-mutation identification using Escherichia coli mismatch-repair detection.
Nature methods - 1 Sept 2007
Peters Brock A, Kan Zhengyan, Sebisanovic Dragan, Pujara Kanan, Wang Zhiyong, Hong Peter, Chow Bernard, Stinson Jeremy, Carlton Victoria E H, Pham Thinh Q, Stern Howard, Waring Paul, Hillan Kenneth J, Eberhard David A, de Sauvage Frederic, Zheng Jianbiao, Faham Malek, Seshagiri Somasekar
Abstract excerpt
The discovery of somatic mutations in cancer tissue is extremely laborious, time-consuming and costly. In an evaluation comparing mismatch repair detection (MRD) against Sanger sequencing for somatic-mutation detection, we found that MRD had a specificity of 96% and a sensitivity of 92%. Our results showed that MRD is a robust and cost-effective alternative to Sanger sequencing for identifying somatic mutations...
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