Article
Enzymatic mutation detection in the P53 gene.
Clinical chemistry - 1 Oct 2000
Inganäs M, Byding S, Eckersten A, Eriksson S, Hultman T, Jorsback A, Löfman E, Sabounchi F, Kressner U, Lindmark G, Tooke N
Abstract excerpt
BACKGROUND: The enzymatic mutation detection (EMD) assay uses the bacteriophage resolvase T4 endonuclease VII, which cleaves preformed heteroduplex molecules at mismatch sites, forming two shorter fragments that can be resolved by gel electrophoresis. The method can be used to detect single and multiple base changes, as well as insertions and deletions. METHODS: The sensitivity, specificity, and positional...
Topics
- Colorectal Neoplasms
- DNA, Complementary
- Endodeoxyribonucleases
- Humans
- Mutation
- Polymerase Chain Reaction
- Sensitivity and Specificity
- Sequence Analysis, DNA
- Tumor Suppressor Protein p53