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Article

A novel method for SNP detection using a new duplex-specific nuclease from crab hepatopancreas.

Genome research - 1 Dec 2002

Shagin Dmitry A, Rebrikov Denis V, Kozhemyako Valery B, Altshuler Ilia M, Shcheglov Alex S, Zhulidov Pavel A, Bogdanova Ekaterina A, Staroverov Dmitry B, Rasskazov Valery A, Lukyanov Sergey

Abstract excerpt

We have characterized a novel nuclease from the Kamchatka crab, designated duplex-specific nuclease (DSN). DSN displays a strong preference for cleaving double-stranded DNA and DNA in DNA-RNA hybrid duplexes, compared to single-stranded DNA. Moreover, the cleavage rate of short, perfectly matched...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 12466298
DOI: 10.1101/gr.547002
PMCID: PMC187582

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-12466298-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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