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Article

FLAG assay as a novel method for real-time signal generation during PCR: application to detection and genotyping of KRAS codon 12 mutations.

Nucleic acids research - 1 Jan 2007

Amicarelli Giulia, Shehi Erlet, Makrigiorgos G Mike, Adlerstein Daniel

Abstract excerpt

Real-time signal generation methods for detection and characterization of low-abundance mutations in genomic DNA are powerful tools for cancer diagnosis and prognosis. Mutations in codon 12 of the oncogene KRAS, for example, are frequently found in several types of human cancers. We have developed a novel real-time PCR technology, FLAG (FLuorescent Amplicon Generation) and adapted it for simultaneously (i)...

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Identifiers

PMID: 17932053
DOI: 10.1093/nar/gkm809
PMCID: PMC2095824

Genes

KRAS

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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