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Article

LigAmp for sensitive detection of single-nucleotide differences.

Nature methods - 1 Nov 2004

Shi Chanjuan, Eshleman Susan H, Jones Dana, Fukushima Noriyoshi, Hua Li, Parker Antony R, Yeo Charles J, Hruban Ralph H, Goggins Michael G, Eshleman James R

Abstract excerpt

We developed the LigAmp assay for sensitive detection and accurate quantification of viruses and cells with single-base mutations. In LigAmp, two oligonucleotides are hybridized adjacently to a DNA template. One oligonucleotide matches the target sequence and contains a probe sequence. If the target sequence is present, the oligonucleotides are ligated together and detected using real-time PCR. LigAmp detected...

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PMID: 15782177
DOI: 10.1038/nmeth713

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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