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Article

Live-cell molecular analysis of Akt activation reveals roles for activation loop phosphorylation.

The Journal of biological chemistry - 14 Dec 2007

Ananthanarayanan Bharath, Fosbrink Matthew, Rahdar Meghdad, Zhang Jin

Abstract excerpt

Activation of the serine/threonine protein kinase Akt/PKB is a multi-step process involving membrane recruitment, phosphorylation, and membrane detachment. To investigate this process in the cellular context, we employed a live-cell fluorescence imaging approach to examine conformational changes of Akt and its membrane association. A fluorescence resonance energy transfer-based reporter of Akt action (ReAktion)...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 17928291
DOI: 10.1074/jbc.M706227200

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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