Article
Mimicking transient activation of protein kinases in living cells.
Proceedings of the National Academy of Sciences of the United States of America - 27 Dec 2016
Klomp Jennifer E, Huyot Vincent, Ray Anne-Marie, Collins Kerrie B, Malik Asrar B, Karginov Andrei V
Abstract excerpt
Physiological stimuli activate protein kinases for finite periods of time, which is critical for specific biological outcomes. Mimicking this transient biological activity of kinases is challenging due to the limitations of existing methods. Here, we report a strategy enabling transient kinase activation in living cells. Using two protein-engineering approaches, we achieve independent control of kinase activation...
Topics
- CSK Tyrosine-Protein Kinase
- Enzyme Activation
- HeLa Cells
- Humans
- Mutation
- Phosphatidylinositol 3-Kinases
- Phosphorylation
- Protein Engineering
- Signal Transduction
- Synthetic Biology